Journal: Biology of reproduction

Article Title: Expression of two isoforms of CD44 in human endometrium.

doi: 10.1095/biolreprod51.4.739

Figure Lengend Snippet: FIG. 1. Immunofluorescence of CD44 in human endometrium. a) Proliferative phase tissue (mAb P3H9) showing immunoreactivity in epithelial cells in a gland as well as in surrounding stroma. b) Decidua of first trimester (mAb P3H9) with strong reactivity in decidual stromal cells. c) Late secretory phase tissue (mAb PIG12) showing lateral staining in gland cells. Gland lumen is to right of the field. d) Nuclear staining in same sections as c. e) Example of tissue (late secretory phase) in which stromal reactivity is present but many glands are unstained (mAb PIG12). Note that one gland at bottom right is immunopositive. f) Nuclear staining, same field as (e). Arrowheads in a, c, and e indicate the apical cell surface of glandular epithelium.

Article Snippet: MATERIALS AND METHODS Antibodies Mouse pan anti-CD44 monoclonal antibodies (mAbs) were as follows: P1G12 and P3H9 (14,15; Chemicon, Temecula, CA); F10-44-2 (Serotec, Oxford, UK); BU52 (The Binding Site, Birmingham, UK); A1G3 (Seralab, High Wycombe, UK); and E1.2, a generous gift from Dr. C. Isacke, Imperial College, London [38].

Techniques: Immunofluorescence, Staining

Journal: Biology of reproduction

Article Title: Expression of two isoforms of CD44 in human endometrium.

doi: 10.1095/biolreprod51.4.739

Figure Lengend Snippet: FIG. 2. Immunofluorescence of CD44 in endometrial epithelial cells (mAb P3H9). al) Freshly isolated gland fragment in confocal microscopy. b) Pri- mary culture at 4 days showing mainly lateral immunoreactivity. c) Ishi- kawa endometrial adenocarcinoma cells with lateral as well as punctate surface staining.

Article Snippet: MATERIALS AND METHODS Antibodies Mouse pan anti-CD44 monoclonal antibodies (mAbs) were as follows: P1G12 and P3H9 (14,15; Chemicon, Temecula, CA); F10-44-2 (Serotec, Oxford, UK); BU52 (The Binding Site, Birmingham, UK); A1G3 (Seralab, High Wycombe, UK); and E1.2, a generous gift from Dr. C. Isacke, Imperial College, London [38].

Techniques: Immunofluorescence, Isolation, Confocal Microscopy, Staining

Journal: Biology of reproduction

Article Title: Expression of two isoforms of CD44 in human endometrium.

doi: 10.1095/biolreprod51.4.739

Figure Lengend Snippet: FIG. 3. Immunoprecipitation of CD44 from surface iodinated gland cells with mAb PIG12 gives a band at approximately 130 kDa. Right lane: con- trol, irrelevant mAb. Dots at right indicate positions of molecular size mark- ers (from the top down) of 200, 116, 67, and 45 kDa.

Article Snippet: MATERIALS AND METHODS Antibodies Mouse pan anti-CD44 monoclonal antibodies (mAbs) were as follows: P1G12 and P3H9 (14,15; Chemicon, Temecula, CA); F10-44-2 (Serotec, Oxford, UK); BU52 (The Binding Site, Birmingham, UK); A1G3 (Seralab, High Wycombe, UK); and E1.2, a generous gift from Dr. C. Isacke, Imperial College, London [38].

Techniques: Immunoprecipitation

Journal: Biology of reproduction

Article Title: Distribution of immune cells in the epididymis of the aging Brown Norway rat is segment-specific and related to the luminal content.

doi: 10.1095/biolreprod61.3.705

Figure Lengend Snippet: FIG. 1. Light micrographs showing sec- tions of the epididymis of the Brown Nor- way rat stained with an antibody for monocytes-macrophages (ED1). A–C) Cor- pus, D–F) distal cauda. A, D) 3 mo, B, E) 18 mo with numerous spermatozoa in the lumen, C, F) 18 mo with occasional sper- matozoa in the lumen. lu, Lumen; it, inter- tubular space; p, principal cells; b, basal cells; c, clear cells; bm, basement mem- brane. Arrows, ED1-positive cells; aster- isks, halo cells. Scale bar, A–F 5 64 mm.

Article Snippet: Mouse anti-rat ED1 (Serotec USA, Washington, DC) was used at a dilution 1/100; this antibody recognizes a cytoplasmic antigen in monocytes and most macrophages.

Techniques: Staining

Journal: Biology of reproduction

Article Title: Distribution of immune cells in the epididymis of the aging Brown Norway rat is segment-specific and related to the luminal content.

doi: 10.1095/biolreprod61.3.705

Figure Lengend Snippet: FIG. 5. Light micrographs showing sec- tions of the corpus of the epididymis of Brown Norway rats. A, C) Three mo; B, D) 18 mo. Section stained with an antibody for A and B GST Yf and C and D ED1. Clear arrows, basal cells; dark arrows, ED1-positive cells; other labels as in Fig- ure 1. Scale bar, A–D 5 64 mm.

Article Snippet: Mouse anti-rat ED1 (Serotec USA, Washington, DC) was used at a dilution 1/100; this antibody recognizes a cytoplasmic antigen in monocytes and most macrophages.

Techniques: Staining

Journal: Cell chemical biology

Article Title: TREM2 is thyroid hormone regulated making the TREM2 pathway druggable with ligands for thyroid hormone receptor.

doi: 10.1016/j.chembiol.2021.07.014

Figure Lengend Snippet: Figure 4. T3 and sobetirome stimulate and NH-3 blocks phagocytosis by microglia (A–D’) C57BL/6 mouse primary microglia cells in culture were treated with DMSO vehicle (A + A’), 10 nM T3 (B + B’), 1 mM sobetirome (C + C’), or 2 mM NH-3 (D + D’) for 24 h before 2 mm diameter fluorescent beads (100/cell) were introduced 2 h before cells were fixed and stained for CD11B (green). (E) Elongated morphology of an unactivated/ramified microglia cell. (F) Morphology of an activated microglia cell upon treatment with sobetirome (see the supplemental information). (G) Three-dimensional view down three separate axes for visualization of beads inside the uppermost cell in the sobetirome treatment group (image generated using Imaris Section function). (H) Quantification of phagocytosed beads per treatment group. Scale bars, 20 mm (A–D), 10–20 mm (A’–D’), and 8–10 mm (E–G), as noted. Statistical significance was determined by a two-tailed, unpaired t test for comparisons between vehicle and group then were plotted together. Asterisks represent significant difference from vehicle unless otherwise noted: *p % 0.05, **p % 0.01, ***p % 0.001. All graphs show mean ± SEM.

Article Snippet: The cells were then permeabilized with PBS containing 0.05% saponin (saponin was included in all subsequent incubations and washes) and stained for microglial marker CD11b using monoclonal rat anti-mouse CD11b (1:200 dilution, AbD Serotec, #MCA711) in conjunction with Alexa Fluor 488-conjugated donkey anti-mouse secondary antibody (1:400 dilution,Thermo Fisher, A21202).

Techniques: Staining, Generated, Two Tailed Test